Accelerate research, empower industry

CRISPR/Cas9-mediated gene knockout (KO)CRISPR/Cas9-mediated gene knockin (KI)CRISPR/Cas9-mediated gene point-mutationInducible Gene Over-expression (Tet-On)shRNA Knockdown cell serviceCRISPR-KO libraryCRISPRa libraryCRISPRi libraryCRISPRSeek™ library screening serviceCRISPRSeek™ Library PlasmidCRISPRSeek™ Library VirusCRISPRSeek™ Library Cell PoolCRISPRSeek™ Library ProductsHySigen Vector LibraryVB163VB164VB203VB204VB205VB303VB271VB272VB188VB278VB180VB251VB252Custom Vector Construction ServiceCustom Vector Construction Service-1Lentivirus PackagingshRNA AAVAdeno-Associated Virus (AAV) PackagingOver-expression AAVIn-stock virusesVirus PackagingCustomized Peptide SynthesisHuman cell linesAnimal cell linesCell LinesKO cell linesPoint mutation cell linesKI cell linesFluorescence-tag cell linesStable cell linesOff-the-shelf gene-edited cell linesOne-step freezing medium for stem cellsOne-step freezing medium for PBMCsCell freezing mediaOsteogenic differentiation culture mediumAdipogenic differentiation culture mediumChondrogenic differentiation culture mediumStem cell differentiation mediaStem cell culture mediaKnockout Cell LinesKnockin Cell LinesPoint mutation cell lineInducible Gene ExpressionGenome-Wide Libraries at Unbeatable PricesWho we arePartnersDistributorsTechnical PlatformsNews & eventsPublicationsResourcesPrivacy policySupportContact UsSubmitted successfully
插件代码
Luciferase Reporter NCI-H1975 Cell Line(NCI-H1975-Luc)
Luciferase Reporter NCI-H1975 Cell Line(NCI-H1975-Luc) 
Price：
$1,260.00
Cat.No.：
Size：
Quantity：
Order
Product info
Documents
References
Cat.No.： TCH-C276L Size： 1x10^6

Product Name：Luciferase Reporter NCI-H1975 Cell Line

HyCyte cell line：HyCyte NCI-H1975-Luc

Catalog No.：TCH-C276L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：NCI-H1975

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin,   100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G276L).

Passage：1:2-1:4

Doubling time：36-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

NCI-H1975 STR profile

Loci

Test Results for Submitted Sample

Sample

Allele1

Allele2

Amelogenin

X


D3S1358

14

15

D5S818

11

12

D2S1338

17


TPOX

8

11

CSF1PO

12


Penta D

12

13

TH01

7


vWA

18


D7S820

8


D21S11

28


Penta E

12

16

D10S1248

13


D8S1179

13

16

D1S1656

14


D18S51

13


D12S391

17


D6S1043

12


D19S433

15

15.2

D16S539

9

12

D13S317

10


FGA

21

24
Contact us
Title
Name
*
Country/Region
*
How would you like us to contact you?
*
E-mail
*
Institution
Phone
Where did you learn about HySigen?
Verification code
Change Image
*
Submit

Quote/Order

We will contact you with information about this product. Please let us know how to reach you.
Contact Us


Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry