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The cell line (originally named 2T) was established in 1964 by Ponten J and Saksela E from a moderately differentiated sarcoma of the tibia in a 15-year-old girl. No viruses were detected through co-cultivation with WI-38 or by complement fixation (CF) assays for anti-simian vacuolating virus SV40, respiratory syncytial virus RSV, or adenovirus. The cells express receptors for insulin-like growth factors I and II (IGF-I, IGF-II) and osteosarcoma-derived growth factor (ODGF).

Cell Name: Human osteosarcoma cell line (STR authenticated)

Cell Synonyms: U-2 OS; U2OS

Catalogue No.: TCH-C365

Species: Human
Tissue Origin: Bone
Disease Characteristic: Osteosarcoma
Morphology: Epithelial-like
Growth Characteristics: Adherent
Culture System: McCoy’s 5A + 10% FBS + 1% P/S
Matching Medium Code: TCH-G365
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma

Manual

Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590

Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590"

Published articles on U2OS

IF=16 Gao Y, Zhu Y, Wang H, et al. Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination[J]. Molecular Cell, 2022, 82(7): 1313-1328. e8.

IF=8.8 Wang W, Yang N, Wang L, et al. The TET-Sall4-BMP regulatory axis controls craniofacial cartilage development[J]. Cell Reports, 2024, 43(3).