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Human Umbilical Vein Endothelial Cell Line 
Cat. code: TCH-C406
Unit size: 1x10^6
Abbreviation: HUVEC
Price:
$1,200.00
General Info
Cell Info
References


Product NameHuman Umbilical Vein Endothelial Cell Line

HyCyte cell lineHUVEC

Catalog No.TCH-C406

OrganismHomo sapiens, human

Organizational SourcesUmbilical cord tissue

MorphologyEndothelial

Growth PropertiesAdherent


Culture System:Endothelial cell basic medium + 5% FBS (fetal bovine serum) + endothelial cell culture supplement + 1% P/S (dual antibiotics). It is recommended to use the HyCyte® complete culture medium for HUVECs , Cat. No. TCH-G406.


Digestion Time:

0.25% trypsin (0.04% EDTA) digests at 37℃ for 1-2 minutes. Note: The digestion time may vary slightly with different brands of trypsin and cell densities, and should be based on the majority of cells rounding up and detaching.


Note:

1. The presence of a small amount of black particles and dead cell debris during cell culture is a normal phenomenon and can be removed by changing the medium.

2. Using HyCyte® complete culture medium, passaging at a ratio of 1:2, it is promised that it can be passaged for an additional five generations after receipt.

3. This cell line is a finite cell line; it is recommended to conduct experiments as soon as possible after receipt, and cryopreservation for preservation is not advised.


Passage Ratio:1:2(based on flask bottom area), Change the fluid every 2-3 days


Culture Environment:Gas phase: 95% air + 5% carbon dioxide, temperature: 37°C


Cryopreservation Conditions:Cryopreservation conditions: 60% basal medium + 30% FBS + 10% DMSO Recommended HyCyte® One Step Freezing Medium, Cat. No. GUCP-R201.

Storage conditions: Liquid nitrogen storage


Safety:

All tumour cells and virus-transfected cells are considered potentially biohazardous, and it is recommended to operate in a biosafety level 2 station with personal protection.


Purpose:

For scientific research purposes only.


IF=9.8 Gao R, Jiang Z, Wu X, et al. Metabolic regulation of tumor cells exposed to different oxygenated polycyclic aromatic hydrocarbons[J]. Science of The Total Environment, 2024, 907: 167833.

IF=8.1 Wang Z, Zhao P, Tian K, et al. TMEM9 promotes lung adenocarcinoma progression via activating the MEK/ERK/STAT3 pathway to induce VEGF expression[J]. Cell Death & Disease, 2024, 15(4): 295.

IF=5.9 Liu Q, Xie J, Zhou R, et al. A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury[J]. Neural Regeneration Research, 2025, 20(2): 503-517.

IF=3.8 Shan Y, Xia Z, An M, et al. Construction and application of H1R ligand screening materials based on SMA stabilization and His-tag covalent immobilization of membrane proteins[J]. Journal of Chromatography A, 2024: 465057.

IF=3.6 Su J, Cheng J, Hu Y, et al. Transfer RNA-derived small RNAs and their potential roles in the therapeutic heterogeneity of sacubitril/valsartan in heart failure patients after acute myocardial infarction[J]. Frontiers in Cardiovascular Medicine, 2022, 9: 961700.

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