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Luciferase Reporter MDA-MB-468 Cell Line(MDA-MB-468-Luc)
Luciferase Reporter MDA-MB-468 Cell Line(MDA-MB-468-Luc) 
Cat. code: TCH-C468L
Unit size: 1x10^6
Abbreviation: HyCyte MDA-MB-468-Luc
Price:
$1,260.00
General Info
Cell Info
References


Product NameLuciferase Reporter MDA-MB-468 Cell Line

HyCyte cell lineHyCyte MDA-MB-468-Luc

Catalog No.TCH-C468L

Construction method:Lentivirus

Selection Marker:Puromycin


ParentalMDA-MB-468

SpeciesHuman

MorphologyEpithelioid

Growth propertiesAdherent

Culture Medium:Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,1.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G468L)

Passage:1:2-1:3

Doubling time:72-96 h

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847

Tumors developed within 21 days at 100% frequency (5/5).

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