Parental:AML12
Species:Mouse
Morphology:Epithelioid
Growth properties:Adherent
Culture Medium:Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,2.0 ug/mL Puromycin,1%ITS,40ng/mL Dexamethasone ,100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCM-G709L).
Passage:1:2-1:4
Doubling time:24-48 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:D/F12+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).