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Product Name：Luciferase Reporter AML12 Cell Line

HyCyte cell line：HyCyte AML12-Luc

Catalog No.：TCM-C709L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：AML12

Species：Mouse

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,2.0 ug/mL Puromycin,1%ITS,40ng/mL Dexamethasone ,100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCM-G709L).

Passage：1:2-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：D/F12+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).