Culture Medium:RPMI-1640 + 10% FBS+ 1% P/S Matching Medium Catalog Number: TCM-G710 Passage Ratio:3×105-5×105 cells/mL, with medium renewal every 2-3 days Doubling Time:—
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
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