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Luciferase Reporter B16-F10 Cell Line(16-F10-Luc)
Luciferase Reporter B16-F10 Cell Line(16-F10-Luc) 
Cat. code： TCM-C713L
Unit size： 1x10^6
Abbreviation： HyCyte B16-F10-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter B16-F10 Cell Line

HyCyte cell line：HyCyte B16-F10-Luc

Catalog No.：TCM-C713L

Construction method：PiggyBac

Selection Marker：Hygro B


Parental：B16-F10

Species：Mouse

Morphology：Epithelioid mixed with spindle cells

Growth properties：Semi-adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G173L）

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790

Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987

Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034

Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927

Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183

Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654

Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469
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