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Luciferase Reporter EL4 Cell Line(EL4-Luc)
Luciferase Reporter EL4 Cell Line(EL4-Luc) 
Cat. code： TCM-C727L
Unit size： 1x10^6
Abbreviation： HyCyte EL4-Luc
Price：
$1,260.00
General Info
Cell Info
References


Product Name：Luciferase Reporter EL4 Cell Line

HyCyte cell line：HyCyte EL4-Luc

Catalog No.：TCM-C727L

Construction method：PiggyBac

Selection Marker：Puromycin


Parental：EL4

Species：Mouse

Morphology：Lymphoblastoid

Growth properties：Suspension

Culture Medium:Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,6.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G727L）

Passage：3×10^5-5×10^5cells/mL

Doubling time： 24-48 h

Subculture： Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).


Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
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