Parental:LL/2 (LLC1)
Species:Mouse
Morphology:Epithelioid
Growth properties:Semi-adherent
Culture Medium:DMEM-H+10%FBS+3.0 ug/mL Puromycin+1%P/S
Passage:1:2-1:4
Doubling time:48-72 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).