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Product Name：Luciferase Reporter MH-S Cell Line

HyCyte cell line：HyCyte MH-S-Luc

Catalog No.：TCM-C795L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：MH-S

Species：Mouse

Morphology：Macrophage

Growth properties：Attached or floating

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0ug/mL Puromycin, 0.05 mM β-mercaptoethanol,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G795L).

Passage：1:2-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).