Parental:A20
Species:Mouse
Morphology:Lymphoblastoid
Growth properties:Suspension
Culture Medium: RPMI-1640 supplemented with 10 % FBS,4.0 ug/mL Puromycin,0.05 mM 2-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G804L).
Passage:3×10^5-5×10^5cells/mL
Doubling time: 24-48 h
Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.
Freezing media: RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).