Parental:4T1.2
Species:Mouse
Morphology:Epithelioid
Growth properties:Adherent
Culture Medium : RPMI-1640+10% FBS+4.0 ug/mL Puromycin+1% P/S
Passage ratio:RPMI-1640 supplemented with 10 % FBS,4.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G810L).
Passage : 1:2-1:4
Doubling time:24-48 h
Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.
Freezing media:RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).