Accelerate research, empower industry

Point Mutation Cell Line


Overview

Cas9-cell line gene mutation (CGM) stands as a highly effective tool for investigating cell gene functions, particularly enabling the in-situ study of point mutations within cells.


Key Advancements

  • Hysigen's Strategies for Single-Base Mutations:

    - Oligo for Short Homology Arms

    - ssDNA for Long Homology Arms

  • Hysigen ensures services are uniquely adapted to the specific requirements of each site and cell line


Our achievements

With a track record of over 100 successful cases, Hysigen has demonstrated proficiency in transfecting commonly used cell lines, including Jurkat, NK-92, BV2, C2C12, EMT6, B16-F10, ARPE-19, HepG2, THP-1, HCT116, A549, RAW264.7, MDA-MB-231, MDA-MB-468, 4T1, and more.



Workflow

MU流程图.jpg

* We kindly remind you that we provide gene editing services for primary cells, stem cells or iPS cells.

Deliverables


Stable Cell line Model

Approach

Cell Type

Price

Turnaround

Deliverables

Point Mutation

RNP homozygous

Easy

$6,999

14-20 weeks

One homozygous single clone, each clone with two vials of cells (>10^6 cells/vial)

Normal

$7,699

18-25 weeks

Difficult

$9,799

23-30 weeks

Technical Information
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CRISPR-mediated point mutation CRISPR-mediated point mutation involves using the CRISPR-Cas9 system to introduce precise changes in a single nucleotide of a targeted gene. The process begins with the design of a guide RNA (gRNA) that directs the Cas9 enzyme to the specific genomic location where the point mutation is desired. The Cas9 protein acts as molecular scissors, creating a double-strand break (DSB) at the target site. The cell's natural repair mechanism, Homology Directed Repair (HDR), then comes into play. HDR can be employed with a donor DNA template containing the desired point mutation. This template guides the precise repair of the DNA, incorporating the desired mutation at the targeted nucleotide position. The CRISPR-mediated point mutation mechanism enables researchers to engineer specific changes in the genome with remarkable precision.

Example


Point mutation of JAK2 in MEG-01 Cells Using CRISPR/Cas9

Experimental design

Strategy


Identification results

PCR screening


Final Clone Sequences

#4C7:

AAGCAGCAAGTATGATGAGCAAGCTTTCTCACAAGCATTTGGTTTTAAATTATGGAGTATGTTTCTGTGGAGACGAGAGTAAGTAAAACTACAGGCTTTCTAATGCCTTTCTCAGAGCAT

* Point mutation: V617F

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Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry