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Overview

We provide the Tet-On system to assist you in achieving precise and tetracycline dose-dependent control over the regulation of your Gene of Interest (GOI) expression.

Key Advancements

• Large insert gene fragment size (up to 30 kb)

• Stable expression for over 20 passages

• Short turnaround

• Inducible target gene expressions with minimal background interference

Workflow

* We kindly remind you that we provide gene editing services for primary cells, stem cells or iPS cells.

PiggyBac with Tet-On system

The PiggyBac transposon integrates the Tet-On system and the target gene into the chromosome of the host cell.

The Tet-On system is a regulated gene expression mechanism, offering precise control over the activation of a target gene. It responds to the presence of tetracycline or its analogs (like Doxycycline). In the “off” state, the Tet-On system remains inactive, and the gene of interest (GOI) is not expressed. Upon introduction of tetracycline or its analogs, the system is triggered to turn “on”, allowing for the controlled and dose-dependent expression of the GOI. This inducible system enables researchers to finely regulate the timing and level of gene expression, offering versatility in experimental designs and facilitating the study of biological processes with a high degree of specificity.