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Technique Information

PiggyBac with Tet-On system

The PiggyBac transposon integrates the Tet-On system and the target gene into the chromosome of the host cell.

The Tet-On system is a regulated gene expression mechanism, offering precise control over the activation of a target gene. It responds to the presence of tetracycline or its analogs (like Doxycycline). In the “off” state, the Tet-On system remains inactive, and the gene of interest (GOI) is not expressed. Upon introduction of tetracycline or its analogs, the system is triggered to turn “on”, allowing for the controlled and dose-dependent expression of the GOI. This inducible system enables researchers to finely regulate the timing and level of gene expression, offering versatility in experimental designs and facilitating the study of biological processes with a high degree of specificity.

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