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Lentivirus packaging services


Recombinant lentivirus is the most commonly used viral vector for efficient gene delivery into mammalian cells. Unlike plasmid DNA vectors, which only enable transient and episomal expression of foreign genes, lentiviral vectors achieve permanent gene expression by integrating into the host cell genome.


Hysigen Biosciences provides lentivirus packaging services with superior quality. We have significantly improved recombinant lentivirus production in terms of titer, purity, viability and consistency, making our lentivirus suitable for a wide range of experimental applications.

Highlights

  • High viral titer: >1×10⁹ TU/ml, validated by standard titer determination assays.

  • Broad tropism: efficient transduction of both dividing and non-dividing cells.

  • Stable expression: Integration into host genome for long-term gene expression.

  • Customizable internal promoters for meeting specific experimental needs.

Experimental validation



A549 cells were transduced by Hysigen EGFP Lentivirus. EGFP expression post tranduction is shown as indicated.


Scale and Turnaround


Services

Scale types

Specifications

Turnaround*

Applications

Over-expression Lentivirus Packaging

Medium

Custom over-expression lentivirus, > x 108 TU/ml, 10 x 100 ul

EGFP control lentivirus, > x 108 TU/ml, 2 x 100 ul (Free)

2-3 weeks

Cell transduction

Large

Custom over-expression lentivirus, > x 109 TU/ml, 10 x 100 ul

EGFP control lentivirus, > x 109 TU/ml, 2 x 100 ul (Free)

2-3 weeks

Cell transduction

Large

ultra-purified

Custom over-expression lentivirus, > x 109 TU/ml, 10 x 100 ul

EGFP control lentivirus, > x 109 TU/ml, 2 x 100 ul (Free)

2-3 weeks

Cell transduction

shRNA Lentivirus Packaging

(Suresilencing 3+1)

Medium

Custom shRNA lentivirus, > x 108 TU/ml, 10 x 100 ul

Scramble control lentivirus, > x 108 TU/ml, 10 x 100 ul (Free)

4-5 weeks

Cell transduction

Medium

ultra-purified   

Custom shRNA lentivirus, > x 109 TU/ml, 10 x 50 ul

Scramble control lentivirus, > x 109 TU/ml, 10 x 50 ul (Free)

4-5 weeks

Cell transduction


      *This does not include the time spent on ORF plasmid construction. Contact our scientists to get more details   for your custom lentivirus packaging.


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Address: 56 Sugar Creek Blvd Suite 375, Sugar Land, TX 77478

Email: info@hysigen.com

Telephone: 628-777-8169 (US)

Accelerate research, empower industry