Hysigen CRISPR Library Virus is developed through the CRISPRSeek™ platform. The process begins with the preparation of library plasmids featuring high coverage and excellent uniformity. These plasmids are then efficiently packaged using a lentiviral packaging kit, after which the supernatant is collected, filtered, and concentrated to generate a high-titer CRISPR library virus.
Leveraging this platform, Hysigen provides CRISPR library viruses that can be directly applied to the construction of stable CRISPR library cell pools, eliminating the need for the time-consuming and complex plasmid amplification step. This greatly streamlines experimental workflows and enhances overall efficiency.