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HySigen CRISPR Library Cell Pool is established through the CRISPRSeek™ platform with a standardized batch production workflow.The process begins with the preparation of high-coverage, high-uniformity library plasmids using HySigen’s proprietary high-efficiency competent cells. These plasmids are then packaged with a lentiviral packaging kit to generate high-titer CRISPR library viruses. Finally, through HySigen’s exclusive cell pool preparation process, the target cell line is infected at a low multiplicity of infection (MOI), ensuring that each cell receives only a single viral integration.Hysigen CRISPR Library Cell Pools demonstrate minimal batch-to-batch variation and excellent reproducibility. By applying different selective pressures to these cell pools, researchers can efficiently identify target genes relevant to a wide range of research fields.