海星生物

AAV Packaging Services

Recombinant adeno-associated virus (AAV) is a versatile and widely utilized viral vector for both in vitro and in vivo gene delivery. It can infect both dividing and non-dividing cells, often requiring the help of adenoviruses or herpes viruses for replication in living organisms. AAVs have become one of the most effective tools for gene therapy due to their ability to transduce a diverse range of mammalian cell types, as well as their non-pathogenic nature and low immunogenicity in humans.

We provides high-quality AAV packaging services to support your gene therapy experiments. We have significantly improved recombinant AAV packaging services in terms of titer, purity, potency, and consistency, making our AAV suitable for a wide range of experimental applications.

Types of AAV provided

Single-stranded AAV

Serotype options include: AAV1, 2, 3b, 4, 5, 6, 6.2, 7, 8, 9, PHP.eB, PHP.B, PHP.S, rh10, DJ, DJ/8, AAV 2-retro, etc.

Highlights

High viral titer:>1×10¹³ GC/ml, validated by standard titer determination assays.
Multiple serotypes available for cell-specific and tissue-specific scenarios.
Low risk of host genome disruption.
Customizable internal promoters for meeting specific experimental needs.
Suitable for in vitro cell culture and in vivo animal models (e.g., mouse, rat).

Technical information

Recombinant AAV (rAAV) acts as a viral vector, combining the AAV2 genome with capsid protein genomes from various serotypes. The rAAV plasmid allows the insertion of coding sequences (CDS) or RNA interference sequences that target specific genes. AAV is mainly administered through in vivo injection due to its low immunogenicity and ability to support long-term gene expression. Our AAV undergoes purification through ultracentrifugation, and the number of genome copies is quantified using quantitative polymerase chain reaction (qPCR). Typically, the AAV titer ranges from 10^12 to 10^13 viral genomes per milliliter (v.g./ml).

Scale and Turnaround

Services	Scale types	Specifications	Turnaround*	Applications
Over-expression AAV Packaging	Medium	Custom AAV, > x 10¹¹ GC/ml, 10 x 100 ul EGFP control AAV, > x 10¹¹GC/ml, 2 x 100 ul (Free)	2-3 weeks	Cell transduction
	Large	Custom AAV , > x 10¹² GC/ml, 10 x 100 ul EGFP control AAV, > x 10¹²GC/ml, 2 x 100 ul (Free)	2-3 weeks	Cell transduction
	Medium ultra-purified	Custom AAV , > x 10¹³ GC/ml, 10 x 50 ul EGFP control AAV, > x 10¹³ GC/ml, 10 x 50 ul (Available for purchase)	2-3 weeks	In-vivo assays
	Large ultra-purified	Custom AAV , > x 10¹³GC/ml, 10 x 100 ul EGFP control AAV, > x 10¹³ GC/ml, 10 x 100 ul ((Available for purchase)	4-6 weeks	In-vivo assays
shRNA AAV Packaging (Suresilencing 3+1)	Medium	Custom AAV, > x 10¹¹ GC/ml, 10 x 100 ul Scramble control AAV, > x 10¹¹ GC/ml, 10 x 100 ul (Free)	4-6 weeks	Cell transduction
	Medium ultra-purified	Custom AAV, > x 10¹³ GC/ml, 10 x 50 ul Scramble control AAV, > x 10¹³ GC/ml, 10 x 50 ul (Free)	4-6 weeks	In-vivo assays
	Large ultra-purified	Custom AAV, > x 10¹³ GC/ml, 10 x 100 ul Scramble control AAV, > x 10¹³GC/ml, 10 x 100 ul (Free)	4-6 weeks	In-vivo assays

*This does not include the time spent on ORF plasmid construction. Contact our scientists to get more details for your custom AAV packaging.

Recommended serotypes for different tissues

Serotypes	Tissues
AAV1	Smooth muscle, skeletal muscle, CNS, brain, lung, retina, inner ear, pancreas, heart, liver
AAV2	Smooth muscle, CNS, brain, liver, pancreas, kidney, retina, inner ear, testes
AAV3b	Smooth muscle, liver, lung
AAV4	CNS, retina, lung, kidney, heart
AAV5	Smooth muscle, CNS, brain, lung, retina, heart
AAV6	Smooth muscle, heart, lung, pancreas, adipose, liver
AAV6.2	Lung. liver, inner ear
AAV7	Smooth muscle, retina, CNS, brain, liver
AAV8	Smooth muscle, CNS, brain, retina, inner ear, liver, pancreas, heart, kidney, adipose
AAV9	Smooth muscle, skeletal muscle, lung, liver, heart, pancreas, CNS, brain, retina, inner ear, testes, kidney, adipose
AAV PHP.eB	CNS
AAV PHP.S	PNS
rh10	Smooth muscle, lung, liver, heart, pancreas, CNS, retina. kidney
DJ	Liver, heart, kidney, spleen
DJ/8	Liver, brain, spleen, kidney
AAV 2-retro	Spinal nerves

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